Fluorescence Study Of Dyes

Part I of Essay
1. Introduction Coumarin 1 and Sodium fluorescein are two dyes which absorb and emit light in the visible region. By using a spectrophotometer we are recording an absorption spectrum and then determining the molar decadic absorption coefficients, which will be used later to ....

Part II of Essay
.... between collisional energy transfer and dipole-dipole transfer. From here we will try to determine the quenching constant and the distance at which decay and energy transfer are equally probable, as well as prove that Stern-Volmer's law and Förster's theory are obeyed. 2. Results 2.1. Electronic Absorption Spectra We made up stock solutions for both salts and diluted them down to use in the UV/vis spectrometer. Coumarin 1 C = 8 x10-5 mol dm -3 We obtained a spectrum with a maximum at 376.5nm at an absorbance of 1.4573 ....